Efficient gene transfers into neocortical neurons connected by NMDA NR1-containing synapses.

ABSTRACT:

Background

Within a circuit, specific neurons and synapses are hypothesized to have essential roles in circuit physiology and learning, and dysfunction in these neurons and synapses causes specific disorders. These critical neurons and synapses are embedded in complex circuits containing many neuron and synapse types.

New method

We established technology that can deliver different genes into pre- and post-synaptic neurons connected by a specific synapse type. The first, presynaptic gene transfer employs standard gene transfer technology to express a synthetic peptide neurotransmitter which has three domains, a dense core vesicle sorting domain for processing the protein as a peptide neurotransmitter, a receptor-binding domain, here a small peptide that binds to NMDA NR1 subu

SUBMITTER: Nagayach A 

PROVIDER: S-EPMC6760849 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature