Ageing affects DNA methylation drift and transcriptional cell-to-cell variability in mouse muscle stem cells.

ABSTRACT: Age-related tissue alterations have been associated with a decline in stem cell number and function. Although increased cell-to-cell variability in transcription or epigenetic marks has been proposed to be a major hallmark of ageing, little is known about the molecular diversity of stem cells during ageing. Here we present a single cell multi-omics study of mouse muscle stem cells, combining single-cell transcriptome and DNA methylome profiling. Aged cells show a global increase of uncoordinated transcriptional heterogeneity biased towards genes regulating cell-niche interactions. We find context-dependent alterations of DNA methylation in aged stem cells. Importantly, promoters with increased methylation heterogeneity are associated with increased transcriptional heterogeneity of the gene

SUBMITTER: Hernando-Herraez I 

PROVIDER: S-EPMC6761124 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature