Development of a qPCR platform for quantification of the five bacteriophages within bacteriophage cocktail 2 (BFC2).

ABSTRACT: To determine phage titers accurately, reproducibly and in a non-laborious and cost-effective manner, we describe the development of a qPCR platform for molecular quantification of five phages present in bacteriophage cocktail 2 (BFC2). We compared the performance of this molecular approach, with regard to quantification and reproducibility, with the standard culture-based double agar overlay method (DAO). We demonstrated that quantification of each of the five phages in BFC2 was possible by means of qPCR, without prior DNA extraction, but yields were significantly higher in comparison to DAO. Although DAO is assumed to provide an indication of the number of infective phage particles, whereas qPCR only provides information on the number of phage genomes, the difference in yield (qPCR/DAO ra

SUBMITTER: Duyvejonck H 

PROVIDER: S-EPMC6761158 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature