High-quality RNA isolation from pigment-rich Dendrobium flowers.

ABSTRACT: Isolation of high-quality RNA from Dendrobium flowers is challenging because of the high levels of pigment, polysaccharides, and polyphenols. In the present study, an efficient CTAB method for RNA extraction from the pigment-rich flowers of Dendrobium was optimised. The optimised method yielded high quantities of RNA (10.1-12.9 µg/g). Spectrophotometric values of A_260/280 in the range of 2.2 to 2.4 and A_260/230 values of 2.0 suggested that the isolated RNA was free of polyphenols, polysaccharides, and protein contaminants. RNA integrity numbers determined by microfluidics were in the range of 7.9-8.9 indicative of intact RNA. In the improved method, the addition of 3 M NaCl and 3% PVP-10 in the extraction buffer, followed by an incubation period of 45 mi

SUBMITTER: Khairul-Anuar MA 

PROVIDER: S-EPMC6761217 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature