Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China.

ABSTRACT: Phosphate solubilizing bacteria (PSB) can convert insoluble forms of phosphorus (P) to accessible forms. Five highly efficient PSB strains, H22, Y11, Y14, Y34, and S32, were screened and isolated from an alfalfa rhizosphere in heavy metal-contaminated reclamation area in Shanxi Province, China. Based on morphological observations, 16S rRNA sequencing, cellular fatty acid composition analysis, and the BIOLOG test, H22, Y11, and Y34 were identified as Pseudomonas sp., while Y14 and S32 were identified as Pantoea sp. Among them, S32 showed the highest P-solubilizing efficiency in culture medium containing Ca₃(PO₄)₂, lecithin, and powered phosphate rock. The culture medium conditions to obtain the highest P-solubilization efficiency were optimized

SUBMITTER: Chen Q 

PROVIDER: S-EPMC6761231 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature