Propagation of Spermatogonial Stem Cell-Like Cells From Infant Boys.

ABSTRACT:

Background

Gonadotoxic treatment of malignant diseases as well as some non-malignant conditions such as cryptorchidism in young boys may result in infertility and failure to father children later in life. As a fertility preserving strategy, several centers collect testicular biopsies to cryopreserve spermatogonial stem cells (SSCs) world-wide. One of the most promising therapeutic strategies is to transplant SSCs back into the seminiferous tubules to initiate endogenous spermatogenesis. However, to obtain sufficient numbers of SSC to warrant transplantation, in vitro propagation of cells is needed together with proper validation of their stem cell identity.

Materials and methods

A minute amount of testicular biopsies (between 5 mg and 10 mg) were processed by mechanic

SUBMITTER: Dong L 

PROVIDER: S-EPMC6761273 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature