Role of MurT C-Terminal Domain in the Amidation of Staphylococcus aureus Peptidoglycan.

ABSTRACT: Glutamate amidation, a secondary modification of the peptidoglycan, was first identified in Staphylococcus aureus It is catalyzed by the protein products of the murT and gatD genes, which are conserved and colocalized in the genomes of most sequenced Gram-positive bacterial species. The MurT-GatD complex is required for cell viability, full resistance to β-lactam antibiotics, and resistance to human lysozyme and is recognized as an attractive target for new antimicrobials. Great effort has been invested in the study of this step, culminating recently in three independent reports addressing the structural elucidation of the MurT-GatD complex. In this work, we demonstrate through the use of nonstructural approaches the critical and multiple roles of the C-terminal domain

SUBMITTER: Goncalves BV 

PROVIDER: S-EPMC6761511 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature