Structure and effective charge characterization of proteins by a mobility capillary electrophoresis based method.

ABSTRACT: Measuring the conformations and effective charges of proteins in solution is critical for investigating protein bioactivity, but their rapid analysis remains a challenging problem. Here we report a mobility capillary electrophoresis (MCE) based method for the rapid analysis of protein stereo-structures and effective charges in different solution environments. With the capability of mixture separation, MCE measures the hydrodynamic radius of a protein through Taylor dispersion analysis and its effective charge through ion mobility analysis. The experimental results acquired from MCE are then utilized to restrain molecular dynamics simulations, so that the most probable conformation of that protein can be obtained. As proof-of-concept demonstrations, the charge states and structures of five

SUBMITTER: Zhang W 

PROVIDER: S-EPMC6761862 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature