Use of fast HPLC multiple reaction monitoring cubed for endogenous retinoic acid quantification in complex matrices.

ABSTRACT: Retinoic acid (RA), an essential active metabolite of vitamin A, controls numerous physiological processes. In addition to the analytical challenges owing to its geometric isomers, low endogenous abundance, and often localized occurrence, nonspecific interferences observed during liquid chromatography (LC) multiple reaction monitoring (MRM) quantification methods have necessitated lengthy chromatography to obtain accurate quantification free of interferences. We report the development and validation of a fast high performance liquid chromatography (HPLC) multiplexing multiple reaction monitoring cubed (MRM(3)) assay for selective and sensitive quantification of endogenous RA from complex matrices. The fast HPLC separation was achieved using an embedded amide C18 column packed with 2.7 μm f

SUBMITTER: Jones JW 

PROVIDER: S-EPMC6761927 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature