RECAP reveals the true statistical significance of ChIP-seq peak calls.

ABSTRACT:

Motivation

Chromatin Immunopreciptation (ChIP)-seq is used extensively to identify sites of transcription factor binding or regions of epigenetic modifications to the genome. A key step in ChIP-seq analysis is peak calling, where genomic regions enriched for ChIP versus control reads are identified. Many programs have been designed to solve this task, but nearly all fall into the statistical trap of using the data twice-once to determine candidate enriched regions, and again to assess enrichment by classical statistical hypothesis testing. This double use of the data invalidates the statistical significance assigned to enriched regions, thus the true significance or reliability of peak calls remains unknown.

Results

Using simulated and real ChIP-seq data, we show that three

SUBMITTER: Chitpin JG 

PROVIDER: S-EPMC6761936 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature