Inter-Modular Linkers play a crucial role in governing the biosynthesis of non-ribosomal peptides.

ABSTRACT:

Motivation

Non-ribosomal peptide synthetases (NRPSs) are modular enzymatic machines that catalyze the ribosome-independent production of structurally complex small peptides, many of which have important clinical applications as antibiotics, antifungals and anti-cancer agents. Several groups have tried to expand natural product diversity by intermixing different NRPS modules to create synthetic peptides. This approach has not been as successful as anticipated, suggesting that these modules are not fully interchangeable.

Results

We explored whether Inter-Modular Linkers (IMLs) impact the ability of NRPS modules to communicate during the synthesis of NRPs. We developed a parser to extract 39 804 IMLs from both well annotated and putative NRPS biosynthetic gene clusters from 39 

SUBMITTER: Farag S 

PROVIDER: S-EPMC6761970 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature