Destin: toolkit for single-cell analysis of chromatin accessibility.

ABSTRACT:

Summary

Single-cell assay of transposase-accessible chromatin followed by sequencing (scATAC-seq) is an emerging new technology for the study of gene regulation with single-cell resolution. The data from scATAC-seq are unique-sparse, binary and highly variable even within the same cell type. As such, neither methods developed for bulk ATAC-seq nor single-cell RNA-seq data are appropriate. Here, we present Destin, a bioinformatic and statistical framework for comprehensive scATAC-seq data analysis. Destin performs cell-type clustering via weighted principle component analysis, weighting accessible chromatin regions by existing genomic annotations and publicly available regulomic datasets. The weights and additional tuning parameters are determined via model-based likelihood. We eval

SUBMITTER: Urrutia E 

PROVIDER: S-EPMC6761983 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature