Intra-individual variation of circulating tumour DNA in lung cancer patients.

ABSTRACT: Circulating tumour DNA (ctDNA) has been increasingly incorporated into the treatment of cancer patients. ctDNA is generally accepted as a powerful diagnostic tool, whereas the utility of ctDNA to monitor disease activity needs to be fully validated. Central to this challenge is the question of whether changes in longitudinal ctDNA measurements reflect disease activity or merely biological variation. Thus, the aim of this study was to explore the intra-individual biological variation of ctDNA in lung cancer patients. We identified tumour-specific mutations using next-generation sequencing. Day-to-day and hour-to-hour variations in plasma concentrations of the mutant allele and wild-type cell-free DNA (cfDNA) were determined using digital PCR. The levels of the mutant alleles varied by as mu

SUBMITTER: Hojbjerg JA 

PROVIDER: S-EPMC6763781 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature