Peptide Assembly Directed and Quantified Using Megadalton DNA Nanostructures.

ABSTRACT: In nature, co-assembly of polypeptides, nucleic acids, and polysaccharides is used to create functional supramolecular structures. Here, we show that DNA nanostructures can be used to template interactions between peptides and to enable the quantification of multivalent interactions that would otherwise not be observable. Our functional building blocks are peptide-oligonucleotide conjugates comprising de novo designed dimeric coiled-coil peptides covalently linked to oligonucleotide tags. These conjugates are incorporated in megadalton DNA origami nanostructures and direct nanostructure association through peptide-peptide interactions. Free and bound nanostructures can be counted directly from electron micrographs, allowing estimation of the dissociation constants of the peptides li

SUBMITTER: Jin J 

PROVIDER: S-EPMC6764022 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature