Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis.

ABSTRACT:

Background

Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes on the chromosome remains challenging in Bacillus subtilis. Thus, developing an efficient and convenient method for B. subtilis multiplex genome editing is imperative.

Results

Here, we developed a CRISPR/Cas9n-based multiplex genome editing system for iterative genome editing in B. subtilis. This system enabled us to introduce various types of genomic modifications with more satisfying efficiency than using CRISPR/Cas9, especially in multiplex gene editing. Our system achieved at least 80% effici

SUBMITTER: Liu D 

PROVIDER: S-EPMC6764132 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature