Glycoprofile Analysis of an Intact Glycoprotein As Inferred by NMR Spectroscopy.

ABSTRACT: Protein N-glycosylation stands out for its intrinsic and functionally related heterogeneity. Despite its biomedical interest, Glycoprofile analysis still remains a major scientific challenge. Here, we present an NMR-based strategy to delineate the N-glycan composition in intact glycoproteins and under physiological conditions. The employed methodology allowed dissecting the glycan pattern of the IgE high-affinity receptor (FcεRIα) expressed in human HEK 293 cells, identifying the presence and relative abundance of specific glycan epitopes. Chemical shifts and differences in the signal line-broadening between the native and the unfolded states were integrated to build a structural model of FcεRIα that was able to identify intramolecular interactions between high-mannose N-glycans and the pr

SUBMITTER: Unione L 

PROVIDER: S-EPMC6764210 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature