Multicenter Study Demonstrates Standardization Requirements for Mold Identification by MALDI-TOF MS.

ABSTRACT:

Objectives

Rapid and accurate mold identification is critical for guiding therapy for mold infections. MALDI-TOF MS has been widely adopted for bacterial and yeast identification; however, few clinical laboratories have applied this technology for routine mold identification due to limited database availability and lack of standardized processes. Here, we evaluated the versatility of the NIH Mold Database in a multicenter evaluation.

Methods

The NIH Mold Database was evaluated by eight US academic centers using a solid media extraction method and a challenge set of 80 clinical mold isolates. Multiple instrument parameters important for spectra optimization were evaluated, leading to the development of two specialized acquisition programs (NIH method and the Alternate-B metho

SUBMITTER: Lau AF 

PROVIDER: S-EPMC6764242 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature