Co-expression network analysis revealing the key lncRNAs in diabetic foot ulcers.

ABSTRACT:

Introduction

Diabetic foot ulcers (DFUs) are the most common foot injuries leading to lower extremity amputation in diabetic patients. Recent studies showed that long non-coding RNAs (lncRNAs) played important roles in diverse biological processes. In this study, we focused on identifying differentially expressed long non-coding RNAs (lncRNAs) in DFU.

Material and methods

Real-time PCR assay was performed to validate the expression pattern of lncRNAs in DFU. Moreover, co-expression networks were also constructed to identify hub lncRNAs in DFU. Specifically, gene ontology (GO) analysis was first performed to evaluate the potential roles of differentially expressed genes (DEGs) and lncRNAs in DFU.

Results

In the present study, we identified 58 up-regulated lncRNAs and 4

SUBMITTER: Yu P 

PROVIDER: S-EPMC6764308 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature