Development and characterization of SSR markers for Sanguinaria canadensis based on genome skimming.

ABSTRACT:

Premise

Polymorphic nuclear simple sequence repeat (nSSR) markers were developed for Sanguinaria canadensis (Papaveraceae), a spring ephemeral native to eastern North America.

Methods and results

Based on the genome skimming data of S. canadensis, a total of 240 nSSR primer pairs were designed for 80 loci from the assembled nuclear contigs. Of these primer pairs, 19 were selected for initial validation in four populations (80 individuals). All 19 loci produced heterologous amplification. The numbers of alleles per locus ranged from one to 21; the levels of observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.847, respectively. Transferability of the loci was tested in the related species Eomecon chionantha.

Con

SUBMITTER: Liao R 

PROVIDER: S-EPMC6764431 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature