Primers for complete chloroplast genome sequencing in Magnolia.

ABSTRACT:

Premise

A new set of primers was developed for sequencing of whole chloroplast genomes of Magnolia species and gap-filling of unfinished genomes.

Methods and results

Two hundred and fifty primers were newly designed based on two previously reported chloroplast genomes from two different genera in Magnoliaceae. A total of 134 primer pairs, including the ones developed in this study and 18 previously reported ones, were enough to cover the entire chloroplast genome sequences in Magnoliaceae. Four species from different sections of Magnolia (M. dealbata, M. fraseri var. pyramidata, M. liliiflora, and M. odora) were used to show the general application of these primers to chloroplast genome sequencing in Magnolia.

Conclu

SUBMITTER: Song E 

PROVIDER: S-EPMC6764489 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature