Protein composition of the outermost exosporium-like layer of Clostridium difficile 630 spores.

ABSTRACT: Clostridium difficile spores are considered the morphotype of infection, transmission and persistence of C. difficile infections. There is a lack of information on the composition of the outermost exosporium layer of C. difficile spores. Using recently developed exosporium removal methods combined with MS/MS, we have established a gel-free approach to analyze the proteome of the exosporium of C. difficile spores of strain 630. A total of 184 proteins were found in the exosporium layer of C. difficile spores. We identified 7 characterized spore coat and/or exosporium proteins; 6 proteins likely to be involved in spore resistance; 6 proteins possibly involved in pathogenicity; 13 uncharacterized proteins; and 146 cytosolic proteins that might have been encased into the exosporium during asse

SUBMITTER: Diaz-Gonzalez F 

PROVIDER: S-EPMC6764588 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature