Validation of microRNA expression profile in Oral Lichenoid Disease through cytological samples.

ABSTRACT:

Background

To validate oral exfoliative cytology in the analysis of the microRNA expression profile in Oral Lichenoid Disease (OLD).

Material and methods

The expression of 13 microRNAs identified and presented by our group in a previous study was analyzed in 26 cases, 16 diagnosed as OLD and 10 controls with no oral mucosal pathology. Cytological samples from the oral mucosa obtained using an Orcellex toothbrush were analyzed using RT-qPCR and TaqMan microRNA assays.

Results

The aberrant expression was validated for 2 microRNAs (miR-146a-5p and miR-7-1-3p) of those previously recognized in the biopsy study.

Conclusions

This is the first time that oral exfoliative cytology is validated in a study of the alterations of the expression of microRNAs in OLD. The alte

SUBMITTER: Setien-Olarra A 

PROVIDER: S-EPMC6764714 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature