Identification and Characterization of Functional Human Monoclonal Antibodies to <i>Plasmodium vivax</i> Duffy-Binding Protein.

ABSTRACT: Plasmodium vivax invasion of reticulocytes relies on distinct receptor-ligand interactions between the parasite and host erythrocytes. Engagement of the highly polymorphic domain II of the P. vivax Duffy-binding protein (DBPII) with the erythrocyte's Duffy Ag receptor for chemokines (DARC) is essential. Some P. vivax-exposed individuals acquired Abs to DBPII that block DBPII-DARC interaction and inhibit P. vivax reticulocyte invasion, and Ab levels correlate with protection against P. vivax malaria. To better understand the functional characteristics and fine specificity of protective human Abs to DBPII, we sorted single DBPII-specific IgG⁺ memory B cells from three individuals with high blocking activity to DBPII. We identified 12 DBPII-specif

SUBMITTER: Carias LL 

PROVIDER: S-EPMC6764764 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature