Single-Molecule Protein Detection in a Biofluid Using a Quantitative Nanopore Sensor.

ABSTRACT: Protein detection in complex biological fluids has wide-ranging significance across proteomics and molecular medicine. Existing detectors cannot readily distinguish between specific and nonspecific interactions in a heterogeneous solution. Here, we show that this daunting shortcoming can be overcome by using a protein bait-containing biological nanopore in mammalian serum. The capture and release events of a protein analyte by the tethered protein bait occur outside the nanopore and are accompanied by uniform current openings. Conversely, nonspecific pore penetrations by nontarget components of serum, which take place inside the nanopore, are featured by irregular current blockades. As a result of this unique peculiarity of the readout between specific protein captures and nonspecific pore

SUBMITTER: Thakur AK 

PROVIDER: S-EPMC6764869 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature