Inhibition of EZH2 Ameliorates Lupus-Like Disease in MRL/lpr Mice.

ABSTRACT:

Objective

We previously identified a role for EZH2, a transcriptional regulator in inducing proinflammatory epigenetic changes in lupus CD4+ T cells. This study was undertaken to investigate whether inhibiting EZH2 ameliorates lupus-like disease in MRL/lpr mice.

Methods

EZH2 expression levels in multiple cell types in lupus patients were evaluated using flow cytometry and messenger RNA expression data. Inhibition of EZH2 in MRL/lpr mice was achieved by intraperitoneal 3'-deazaneplanocin (DZNep) administration using a preventative and a therapeutic treatment model. Effects of DZNep on animal survival, anti-double-stranded DNA (anti-dsDNA) antibody production, proteinuria, renal histopathology, cytokine production, and T and B cell numbers and percentages were assessed.

Res

SUBMITTER: Rohraff DM 

PROVIDER: S-EPMC6764871 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature