Live cell optical assay for precise characterization of receptors coupling to Gα12.

ABSTRACT: Heterotrimeric G proteins are essential mediators of G protein-coupled receptors (GPCRs) signalling to intracellular effectors. There is a considerable diversity of G protein subunits that channel signals initiated by GPCRs into specific outcome. In particular, mammalian genomes contain 16 conserved genes encoding G protein α subunits with unique properties. Of four Gα subfamilies (Gi/o, Gq, Gs and G12/13), members of the G12/13 group have received considerable attention for their roles in carcinogenesis. However, our ability to study activation of G12/13 by GPCRs with the power to distinguish between the two subunits is limited. Here, we present an adaptation of the bioluminescence resonance energy transfer (BRET)-based assay to specifically monitor activity of Gα12 in living cells. In th

SUBMITTER: Masuho I 

PROVIDER: S-EPMC6764917 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature