A 'poly-transfection' method for rapid, one-pot characterization and optimization of genetic systems.

ABSTRACT: Biological research is relying on increasingly complex genetic systems and circuits to perform sophisticated operations in living cells. Performing these operations often requires simultaneous delivery of many genes, and optimizing the stoichiometry of these genes can yield drastic improvements in performance. However, sufficiently sampling the large design space of gene expression stoichiometries in mammalian cells using current methods is cumbersome, complex, or expensive. We present a 'poly-transfection' method as a simple yet high-throughput alternative that enables comprehensive evaluation of genetic systems in a single, readily-prepared transfection sample. Each cell in a poly-transfection represents an independent measurement at a distinct gene expression stoichiometry, fully levera

SUBMITTER: Gam JJ 

PROVIDER: S-EPMC6765116 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature