Evolution-based screening enables genome-wide prioritization and discovery of DNA repair genes.

ABSTRACT: DNA repair is critical for genome stability and is maintained through conserved pathways. Traditional genome-wide mammalian screens are both expensive and laborious. However, computational approaches circumvent these limitations and are a powerful tool to identify new DNA repair factors. By analyzing the evolutionary relationships between genes in the major DNA repair pathways, we uncovered functional relationships between individual genes and identified partners. Here we ranked 17,487 mammalian genes for coevolution with 6 distinct DNA repair pathways. Direct comparison to genetic screens for homologous recombination or Fanconi anemia factors indicates that our evolution-based screen is comparable, if not superior, to traditional screening approaches. Demonstrating the utility of our stra

SUBMITTER: Brunette GJ 

PROVIDER: S-EPMC6765268 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature