Cloning expression and immunogenicity analysis of inhibin gene in Ye Mule Aries sheep.

ABSTRACT:

Background

Ye Mule Aries sheep is one of the most important sheep breeds in Xinjiang, China. This breed is well adapted to harsh environmental conditions and displays strong disease resistance, fast growth, and high cold tolerance. To analyze the clonal expression and immunogenicity of the Ye Mule Aries sheep inhibin gene, total RNA was extracted from sheep ovarian tissue and used as a template to generate a eukaryotic expression vector and study inhibin immunogenicity.

Methods

Primers were designed to amplify the inhibin A gene via polymerase chain reaction and the amplified product was cloned between the ScalI and EcoRI restriction sites of the expression vector pEGFP-N1 to construct a recombinant plasmid, pEGFP-INHα. Following the validation of successful cl

SUBMITTER: Huang Z 

PROVIDER: S-EPMC6765352 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature