Development of a highly sensitive magneto-enzyme lateral flow immunoassay for dengue NS1 detection.

ABSTRACT:

Background

Dengue infection represents a global health issue of growing importance. Dengue non-structural protein 1 (NS1) plays a central role in the early detection of the disease. The most common method for NS1 detection is testing by lateral flow immunoassays (LFIAs) with varying sensitivity. In this study, we present a highly sensitive magneto-enzyme LFIA for prompt diagnosis of dengue.

Methods

We have demonstrated the development of a magneto-enzyme LFIA combining super-paramagnetic nanoparticles as labels and Biotin-Streptavidin signal amplification strategy to detect dengue NS1. Factors affecting the test performance including antibody pair, super-paramagnetic nanoparticle size, nitrocellulose membrane type, amounts of detection and capture antibodies, and amounts of

SUBMITTER: Tran TV 

PROVIDER: S-EPMC6765353 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature