Genetic diversity, functional properties and expression analysis of NnSBE genes involved in starch synthesis of lotus (Nelumbo nucifera Gaertn.).

ABSTRACT:

Background

Starch branching enzyme (SBE) is one of the key enzymes in starch biosynthetic metabolism, determining amylopectin structure.

Methods

Full length coding sequences (CDS) of SBE genes were cloned using reverse transcription PCR (RT-PCR) technology, and neighbor-joining (NJ) tree was used for phylogenetic analysis. Single nucleotide polymorphisms (SNPs) were determined to assess the genetic polymorphisms and variation indexes between individuals and clusters. Quantitative real time PCR (qRT-PCR) was performed to analyze the spatial and temporal expression of NnSBE genes. The effect of NnSBE genes on amylopectin's fine structures was explored using affinity and the enzyme activity analysis of two isoforms in amylopectin and amylose.

Results

SUBMITTER: Zhu F 

PROVIDER: S-EPMC6765360 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature