Serine proteases as luminal mediators of intestinal barrier dysfunction and symptom severity in IBS.

ABSTRACT:

Objective

The intestinal lumen contains several proteases. Our aim was to determine the role of faecal proteases in mediating barrier dysfunction and symptoms in IBS.

Design

39 patients with IBS and 25 healthy volunteers completed questionnaires, assessments of in vivo permeability, ex vivo colonic barrier function in Ussing chambers, tight junction (TJ) proteins, ultrastructural morphology and 16 s sequencing of faecal microbiota rRNA. A casein-based assay was used to measure proteolytic activity (PA) in faecal supernatants (FSNs). Colonic barrier function was determined in mice (ex-germ free) humanised with microbial communities associated with different human PA states.

Results

Patients with IBS had higher faecal PA than healthy volunteers. 8/20 postinfection IBS (

SUBMITTER: Edogawa S 

PROVIDER: S-EPMC6765451 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature