[High-throughput sequencing for analysis of structural change of intestinal microbiota in patients with colorectal adenoma].

ABSTRACT: OBJECTIVE:To investigate the taxonomic richness and diversity of gut microbiota in patients with colorectal adenoma and elucidate the role of gut microorganisms in precancerous lesions in the colon and rectum. METHOD:Adenomatous tissues from 31 patients with colorectal adenoma and normal intestinal mucosal tissues from 20 healthy control subjects were collected through colonoscopy. The total bacterial genomic DNA was extracted, and the V3-V4 hypervariable region in bacterial 16S rRNA gene was amplified using polymerase chain reaction and sequenced on an Illumina MiSeq platform. RESULTS:Patients with colorectal adenomas had a higher alpha diversity and richness indices compared to the healthy controls (P<0.01). The mucosal microbiota in colorectal adenoma tissue showed a distinctive structu

SUBMITTER: Lu YY 

PROVIDER: S-EPMC6765500 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature