[Small interfering RNA-mediated programmed cell death-ligand 1 silencing in human glioma cells enhances human CD8⁺ T lymphocyte cytotoxicity in vitro].

ABSTRACT:

Objective

To investigate the effect of small interfering RNA (siRNA)-mediated silencing of programmed cell deathligand 1 (PD-L1) in human glioma cells on the cytotoxicity of human CD8⁺T lymphocytes against the modified tumor cells.

Methods

A siRNA sequence targeting PD-L1 gene was designed and transfected into human glioma U87 MG cells via lipofectamine 2000, and the gene silencing effect was validated using RT-qPCR, Western blotting, and flow cytometry. The transfected cells were co-cultured with human CD8⁺T lymphocytes, and the apoptosis of the tumor cells was analyzed with flow cytometry.

Results

The siRNA sequence showed strong PD-L1 gene-silencing effect at both mRNA and protein levels in U87 MG cells. Compared with the control cells, the tr

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6765535 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature