Characterization and determination of cadmium resistance of CD133⁺/CD24⁺ and CD133^-/CD24⁺ cells isolated from the immortalized human proximal tubule cell line, RPTEC/TERT1.

ABSTRACT: Stem/progenitor cells are involved in the regeneration of the renal tubules after damage due to a toxic insult. However, the mechanism involved in the regeneration of the tubules by the stem cells is not well understood due to the lack of immortal cell lines that represent the stem/progenitor cells of the kidney. A previous study from our laboratory has shown that the immortalized cell line RPTEC/TERT1 contains two populations of cells, one co-expressing CD24 and CD133, the other expressing CD24 only. The goal of the present study was to determine if both these populations could be sorted into separate independent cultures and if so, determine their characteristic features and response to the nephrotoxicant cadmium. The results of our study show that both the populations of cells could gro

SUBMITTER: Shrestha S 

PROVIDER: S-EPMC6766375 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature