Functional genomics of CDHR3 confirms its role in HRV-C infection and childhood asthma exacerbations.

ABSTRACT:

Background

Research in transformed immortalized cell lines indicates the cadherin-related family member 3 (CDHR3) protein serves as a receptor for human rhinovirus (HRV)-C. Similar experiments indicate that the CDHR3 coding variant rs6967330 increases CDHR3 protein surface expression.

Objective

We sought to determine whether CDHR3 is necessary for HRV-C infection of primary airway epithelial cells (AECs) and to identify molecular mechanisms by which CDHR3 variants confer risk for asthma exacerbations.

Methods

CDHR3 function and influence on HRV-C infection were investigated by using single-cell transcriptomics, CRISPR-Cas9 gene knockout, and genotype-specific donor experiments performed in primary AECs. Nasal airway epithelium cis-expression quantitative trait locus (

SUBMITTER: Everman JL 

PROVIDER: S-EPMC6766426 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature