The gut microbiome alters immunophenotype and survival from sepsis.

ABSTRACT: The microbiome is increasingly implicated in immune regulation and mortality from sepsis. Mice with identical genetic backgrounds but distinct microbiomes were obtained from different vendors and analyzed following cecal ligation and puncture (CLP). β diversity of the microbiome measured from feces demonstrated significant differences between The Jackson Laboratory (Jax; Bar Harbor, ME, USA) and Charles River Laboratories (CR; Wilmington, MA, USA) C57/B6 mice. Jax mice had 7-d mortality of 90% following CLP, whereas CR mice had a mortality of 53%. Differences in vendor were associated with altered immunophenotype with increased splenic IFN-γ⁺CD4⁺ T cells, effector memory CD4⁺ T cells, and central memory CD4⁺ T cells and increased Peyer's patch ef

SUBMITTER: Fay KT 

PROVIDER: S-EPMC6766641 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature