In vitro and in vivo investigation of chlorophyll binding sites involved in non-photochemical quenching in Chlamydomonas reinhardtii.

ABSTRACT: Non-photochemical quenching (NPQ) of the light energy absorbed is one of the main photoprotective mechanisms evolved by oxygenic photosynthetic organisms to avoid photodamage, at a cost of reduced photosynthetic efficiency. Tuning of NPQ has been reported as a promising biotechnological strategy to increase productivity in both higher plants and unicellular microalgae. Engineering of NPQ induction requires the comprehension of its molecular mechanism(s), strongly debated in the last three decades with several different models proposed. In this work, the molecular details of NPQ induction was investigated at intramolecular level by in vitro and in vitro site-specific mutagenesis on chlorophyll binding sites of the Light-Harvesting Complex Stress-Related 3 (LHCSR3) protein, the pigment bindi

SUBMITTER: Perozeni F 

PROVIDER: S-EPMC6767442 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature