Defining the regulon of genes controlled by σ^E , a key regulator of the cell envelope stress response in Streptomyces coelicolor.

ABSTRACT: The extracytoplasmic function (ECF) σ factor, σ^E , is a key regulator of the cell envelope stress response in Streptomyces coelicolor. Although its role in maintaining cell wall integrity has been known for over a decade, a comprehensive analysis of the genes under its control has not been undertaken. Here, using a combination of chromatin immunoprecipitation-sequencing (ChIP-seq), microarray transcriptional profiling and bioinformatic analysis, we attempt to define the σ^E regulon. Approximately half of the genes identified encode proteins implicated in cell envelope function. Seventeen novel targets were validated by S1 nuclease mapping or in vitro transcription, establishing a σ^E -binding consensus. Subsequently, we used bioinformatic analysis to look for

SUBMITTER: Tran NT 

PROVIDER: S-EPMC6767563 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature