Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in Trichoderma reesei.

ABSTRACT:

Background

The filamentous ascomycete T. reesei is industrially used to produce cellulases and xylanases. Cost-effective production of cellulases is a bottleneck for biofuel production. Previously, different strain and process optimizations were deployed to enhance enzyme production rates. One approach is the overexpression of the main activator Xyr1 and a second is the construction of synthetic transcription factors. Notably, these genetic manipulations were introduced into strains bearing the wild-type xyr1 gene and locus.

Results

Here, we constructed a Xyr1-deficient strain expressing a non-functional truncated version of Xyr1. This strain was successfully used as platform strain for overexpression of Xyr1, which enhanced the cellulase and xylanase productio

SUBMITTER: Derntl C 

PROVIDER: S-EPMC6767844 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature