Ontology highlight
ABSTRACT:
SUBMITTER: Thevathasan JV
PROVIDER: S-EPMC6768092 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Thevathasan Jervis Vermal JV Kahnwald Maurice M Cieśliński Konstanty K Hoess Philipp P Peneti Sudheer Kumar SK Reitberger Manuel M Heid Daniel D Kasuba Krishna Chaitanya KC Hoerner Sarah Janice SJ Li Yiming Y Wu Yu-Le YL Mund Markus M Matti Ulf U Pereira Pedro Matos PM Henriques Ricardo R Nijmeijer Bianca B Kueblbeck Moritz M Sabinina Vilma Jimenez VJ Ellenberg Jan J Ries Jonas J
Nature methods 20190927 10
Quantitative fluorescence and superresolution microscopy are often limited by insufficient data quality or artifacts. In this context, it is essential to have biologically relevant control samples to benchmark and optimize the quality of microscopes, labels and imaging conditions. Here, we exploit the stereotypic arrangement of proteins in the nuclear pore complex as in situ reference structures to characterize the performance of a variety of microscopy modalities. We created four genome edited ...[more]