Functional and structural characterization of HspB1/Hsp27 from Chinese hamster ovary cells.

ABSTRACT: Small heat shock proteins (sHsps) endow cells with stress tolerance. Of the various sHsps in mammals, HspB1, also known as Hsp27, is the most ubiquitous. To examine the structure and function of HspB1, we expressed, purified, and characterized HspB1 from Chinese hamster (Cricetulus griseus) ovary cells (CgHspB1). CgHspB1 forms a large oligomeric structure. We observed a monodisperse 16-mer with an elongated sphere, but this is affected by changes in various conditions, including temperature. Under dilute conditions, CgHspB1 dissociates into small oligomers at elevated temperatures. The dissociated conformers interacted with the gel filtration column through hydrophobic interactions. In contrast, dissociation of the oligomer was not observed by small-angle X-ray scattering at 55 °C. The res

SUBMITTER: Sha E 

PROVIDER: S-EPMC6768103 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature