Influence of glucose and insulin in human adipogenic differentiation models with adipose-derived stem cells.

ABSTRACT: Autologous fat grafting represents an attractive source for tissue engineering applications in the field of reconstructive medicine. However, in adipogenic differentiation protocols for human adipose-derived stem cells, the concentration of glucose and insulin varies considerably. With the intent to gain maximum tissue augmentation, we focused on the late phase of adipogenesis. In this study, we modified the differentiation protocol for adipose-derived stem cells by prolongation of the induction period and the application highly concentrated glucose and insulin. Human adipose-derived stem cells were isolated from subcutaneous depots and differentiated in a standard induction medium for the first two weeks, followed by two weeks with varying glucose and insulin concentrations. Morphological

SUBMITTER: Kolodziej M 

PROVIDER: S-EPMC6768274 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature