The Metastasis Suppressor Protein Nme1 Is a Concentration-Dependent Modulator of Ca²⁺/Calmodulin-Dependent Protein Kinase II.

ABSTRACT: Nucleoside diphosphate kinases (Nmes or NDPKs) have been implicated in a multitude of cellular processes, including an important role in metastasis suppression, and several enzymatic activities have been assigned to the Nme family. Nevertheless, for many of these processes, it has not been possible to establish a strong connection between Nme enzymatic activity and the relevant biological function. We hypothesized that, in addition to its known enzymatic functions, members of the Nme family might also regulate signaling cascades by acting on key signal transducers. Accordingly, here we show that Nme1 directly interacts with the calcium/calmodulin-dependent kinase II (CaMKII). Using purified proteins, we monitored the phosphorylation of a number of CaMKII substrates and determined that at n

SUBMITTER: Royer L 

PROVIDER: S-EPMC6768392 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature