Cinobufacini ameliorates experimental colitis via modulating the composition of gut microbiota.

ABSTRACT:

Background

Cinobufacini, the sterilized hot water extraction of dried toad skin, has been widely used in the treatment of inflammation and cancers. Recently we found cinobufacini could ameliorate dextran sulfate sodium (DSS)-induced colitis in mice, but the underlying mechanism was not fully understood. In current study, we explored the effect of cinobufacini on gut microbiota in DSS-induced acute colitic mouse model by pyrosequencing of colonic contents.

Methods

C57BL/6 mice were supplied with normal or 3.0% DSS containing drinking water. DSS-treat mice were gavaged daily either with vehicle (water) or cinobufacini (10.0 or 30.0 mg/kg) for 7 days. The composition of the gut microbiota was assessed by analyzing 16S rRNA gene sequences.

Results

Our data indicated that

SUBMITTER: Bai Y 

PROVIDER: S-EPMC6768468 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature