Multiple Long-Read Sequencing Survey of Herpes Simplex Virus Dynamic Transcriptome.

ABSTRACT: Long-read sequencing (LRS) has become increasingly important in RNA research due to its strength in resolving complex transcriptomic architectures. In this regard, currently two LRS platforms have demonstrated adequate performance: the Single Molecule Real-Time Sequencing by Pacific Biosciences (PacBio) and the nanopore sequencing by Oxford Nanopore Technologies (ONT). Even though these techniques produce lower coverage and are more error prone than short-read sequencing, they continue to be more successful in identifying polycistronic RNAs, transcript isoforms including splice and transcript end variants, as well as transcript overlaps. Recent reports have successfully applied LRS for the investigation of the transcriptome of viruses belonging to various families. These studies have subst

SUBMITTER: Tombacz D 

PROVIDER: S-EPMC6769088 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature