A Computational Pipeline for the Extraction of Actionable Biological Information From NGS-Phage Display Experiments.

ABSTRACT: Phage Display is a powerful method for the identification of peptide binding to targets of variable complexities and tissues, from unique molecules to the internal surfaces of vessels of living organisms. Particularly for in vivo screenings, the resulting repertoires can be very complex and difficult to study with traditional approaches. Next Generation Sequencing (NGS) opened the possibility to acquire high resolution overviews of such repertoires and thus facilitates the identification of binders of interest. Additionally, the ever-increasing amount of available genome/proteome information became satisfactory regarding the identification of putative mimicked proteins, due to the large scale on which partial sequence homology is assessed. However, the subsequent production of massi

SUBMITTER: Vekris A 

PROVIDER: S-EPMC6769401 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature