Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide.

ABSTRACT: Cell-penetrating peptides (CPPs) are short peptides that can translocate and transport cargoes into the intracellular milieu by crossing biological membranes. The mode of interaction and internalization of cell-penetrating peptides has long been controversial. While their interaction with anionic membranes is quite well understood, the insertion and behavior of CPPs in zwitterionic membranes, a major lipid component of eukaryotic cell membranes, is poorly studied. Herein, we investigated the membrane insertion of RW16 into zwitterionic membranes, a versatile CPP that also presents antibacterial and antitumor activities. Using complementary approaches, including NMR spectroscopy, fluorescence spectroscopy, circular dichroism, and molecular dynamic simulations, we determined the high-resolut

SUBMITTER: Jobin ML 

PROVIDER: S-EPMC6769507 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature