Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering.

ABSTRACT: Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scatte

SUBMITTER: Bozic D 

PROVIDER: S-EPMC6769602 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature