Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii.

ABSTRACT: Autophagy is a highly conserved catabolic process in eukaryotic cells by which waste cellular components are recycled to maintain growth in both favorable and stress conditions. Autophagy has been linked to lipid metabolism in microalgae; however, the mechanism underlying this interaction remains unclear. In this study, transgenic Chlamydomonas reinhardtii cells that stably express the red fluorescent protein (mCherry) tagged-ATG8 as an autophagy marker were established. By using this tool, we were able to follow the autophagy process in live microalgal cells under various conditions. Live-cell and transmission electron microscopy (TEM) imaging revealed physical contacts between lipid droplets and autophagic structures during the early stage of nitrogen starvation, while fusion of these tw

SUBMITTER: Tran QG 

PROVIDER: S-EPMC6769876 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature